The Position:

The Laboratory Assistant (LA) will be involved in protein expression and purification, protein-ligand binding studies, and general laboratory procedures involving molecular biology. The LA will also be involved in executing and evaluating results from pre-clinical animal models of allergy and asthma. Research projects will cover the discovery process at multiple levels from the production and purification of novel therapeutic target to evaluation of early-stage leads in in vivo projects. The LA will be completely aligned with BTI’s entrepreneurial culture fueled by innovation and growth that is encapsulated in four fundamental pillars: 1) Be bold and decisive, 2) Never give up, 3) Be solution-oriented and 4) Do the impossible.

Key Responsibilities:

• Laboratory Procedures: Perform various standard experimental assays including protein expression using a variety of biological systems, protein purification and characterization, quantitative real-time PCR, western blot, flow cytometry, and ELISA. Work in sterile conditions to conduct cell culture experiments and maintain the growth of cell lines. Collect and analyze generated data. Prepare sterile buffers and media for experiments. Label tubes for specimens collection and update sample inventories.
• Laboratory Maintenance: Assist in organization and upkeep of laboratory supplies, workspace, and equipment in line with standard dictated by the leadership. Sterilization of supplies and waste by autoclave. Develop standard operating procedures.
• Animal Procedures: Conduct various IACUC-approved laboratory animal procedures and techniques for a wide range of projects including but not limited to basic rodent and pig handling, oral gavage, intraperitoneal injection, blood collection, and euthanasia.
• Personnel Training: Training and supervising of personnel on experimental assays and research projects.
• Regulatory compliance: Support regulatory compliance efforts as instructed by the Lab Manager and senior leadership in accordance with IACUC, IBC, and IRB protocols.
• Genotyping: Aid in the genotyping process including tag & snip, PCR, gel electrophoresis, documentation of genotypes and strain histories.
• Teamwork: Work independently and collaboratively, including managing others in a matrixed environment. Be able to successfully work with other team members in experiments and laboratory upkeep. Act in accordance with Company’s policies.
• Communication and reporting: Keep detailed records of all activities, record these activities in ASANA, Google Drive, laboratory notebook, the Colony Management System (CMS), and Labkey. Provide weekly and bi-weekly updates of the research progress to the Scientific Director, research scientists, and Senior Leadership team. Publish original results of research in peer-reviewed, high-impact scientific journals. Maintain the confidentiality of all company deliberations and plans.
• Any new responsibilities or changes in the reporting structure as directed by the leadership.
• Team-science oriented and data-driven, engendering credibility and confidence within and outside the company.
• Maintain the confidentiality of all company deliberations and plans.
• Any new responsibilities or changes in the reporting structure as directed by the leadership.
• Act in accordance with Company’s policies and in alignment with our corporate culture.

Job Experience:
Important skills:

• Passion for teamwork, immense energy, absolute integrity, and an A+ attitude.
• Organized with strong written and verbal communication skills
• Familiarity with laboratory and research practices.

• A+ attitude, great work ethic, and attention to detail
• Robust initiative, passion for BTI.
• Mouse handling and genotyping
• Laboratory assays: real-time PCR, Western blot, and ELISA
• SOP development, record keeping skills
• Experience implementing murine models of immune-mediated and infectious diseases
• Familiarity with drug development process highly desired
• Knowledge of mucosal immunity and inflammatory processes
• Broad range of analytical chemistry skills
• Sample processing for sequencing applications
• Multi-parameter flow cytometry staining and analysis
• Analytical software: PRISM, FACS Diva, FlowJo, Excel, SAS, IPA
• Demonstrated experience analyzing, interpreting and presenting experimental data and developing reports
• IACUC, IBC, and IRB protocol writing experience